Total Cholesterol and Cholesterol species determination
نویسندگان
چکیده
منابع مشابه
Enzymatic determination of total serum cholesterol.
An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the Calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-o...
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In Iran,the hydrogenated vegetable oil have content of frans fatty acids and high per capita consumption.this study was aimed to evaliate the effects of substitution of hydrogenated oil by sunflower liquid oil on plasma levels of LDL,HDL, total cholestero and triglycerids in normal subjects.thirty six healthy inmates participated in this research. they were consuming hydrogenated oil before the...
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We describe a continuous-flow, automated determination of total cholesterol in serum, which is based on enzymatic hydrolysis of cholesterol esters, oxidation of cholesterol by cholesterol oxidase, and colorimetric measurement of liberated perioxide with 4-aminoantipyrine, phenol, and peroxidase. Free cholesterol is determined with the same AutoAnalyzer II manifold and reagents, except that chol...
متن کاملDetermination of total and esterified cholesterol in serum.
A simple method for the determination of total and esterified cholesterol is described. Serum lipids are extracted into a solvent mixture and aliquots of the extract are analyzed directly for free and total cholesterol by the Zlatkis reaction. Esterified cholesterol is calculated by difference. Free cholesterol is determined under conditions of temperature and solvent composition such that the ...
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Since the Liebermann-Burchard reaction was adopted by Grigaut (1) for the quantitative determination of serum total cholesterol, many modified procedures of the original method have been made. The various procedures have differed in extraction method and in the reagents used in the color development. The separation of cholesterol from its esters with digitonin has had limited application becaus...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2011
ISSN: 2043-0116
DOI: 10.1038/protex.2011.245